Cre/loxP system C（3〜6か月） Nestin-creトランスジェニックマウス。Cre-loxP組換え検出：全脳、脊髄、精巣/卵巣 (標的遺伝子のfloxおよびNestin-Creをもつマウスでは、floxとなっている標的遺伝子が精巣/卵巣でCre-loxP組み換えを起こす) 、未検出：胸腺、肺、心臓、肝臓、腎臓、脾臓、医、小腸、大脳、膵臓、骨格筋、尻尾 (開発者情報) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature(s) designated by the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of academic research. Prior to publication, the RECIPIENT should contact the developer scientist, Dr. Shigeru Noguchi and acknowledge Drs. Shigeru Noguchi and Dr. Toshikuni Sasaoka. 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。公表を前提とした学術研究に限る。公表にあたっては、代表開発者の野口茂先生に確認の上、野口茂博士(Dr. Shigeru Noguchi)及び笹岡俊邦博士(Dr. Toshikuni Sasaoka)に対して謝辞を述べる。 Koichiro NISHIMURA 明治乳業ヘルスサイエンス研究所 兼 神奈川科学技術アカデミー・野口茂先生、新潟大学脳研究所生命科学リソース研究センター・笹岡俊邦先生。B6D2F1受精卵へTgのインジェクションにより作出。C57BL/6背景。 true 西村　浩一郎 B6.Cg-Tg(Nes-cre)1Nogu B6.Cg-Tg(Nes-cre)1Nogu B6.Cg-Tg(Nes-cre)1Nogu B6.Cg-Tg(Nes-cre)1Nogu Developed by Dr. Shigeru Noguchi, Meiji Dairies Corporation and Kanagawa Academy of Science and Technology and Dr. Toshikuni Sasaoka, Center for Bioresource-based Researches, Brain Research Institute, Niigata University. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The mice were backcrossed to C57BL/6. Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> C (3-6 months) RBRC02412 Nestin-creトランスジェニックマウス。Cre-loxP組換え検出：全脳、脊髄、精巣/卵巣 (標的遺伝子のfloxおよびNestin-Creをもつマウスでは、floxとなっている標的遺伝子が精巣/卵巣でCre-loxP組み換えを起こす) 、未検出：胸腺、肺、心臓、肝臓、腎臓、脾臓、医、小腸、大脳、膵臓、骨格筋、尻尾 (開発者情報) , Fluorescent Proteins/lacZ System Rat nestin genomic DNA, phage P1 cre recombinase, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires) sequence, Simian virus 40 Large T antigen nuclear localization signal (NLS), E. coli lacZ, SV40 poly A Nestin-cre transgenic mice, expressing a cre recombinase gene under the control of Nestin promoter and enhancer. This Nestin-cre transgene contains a rat nestin promoter, cre recombinase gene, IRES-lacZ-polyA and neuron specific enhancer (around 100 bp of exon 1, the entire intron 1 and around 100 bp of exon 3 of nesitin gene). Tissue-specific expression: observed in whole brain, spinal cord and testis/ovary; not observed in thymus, lung, heart, kidney, spleen, stomach, small and large intestine, pancreas, skeletal muscle and tail (Developers, personal communication). <a href='https://brc.riken.jp/mus/pcr02412'>Genotyping protocol -PCR-</a> Backcross to C57BL/6 (Hemizygote x C57BL/6JJcl) Backcross to C57BL/6 (Hemizygote x C57BL/6JJcl)